NBS-LRR-containing class of salicylic acid-induced gene transcript in rye
نویسندگان
چکیده
NBS-LRR-type disease resistance gene-like cDNA, induced by salicylic acid (SA) was cloned from rye Secalecereale L. (2n = 14RR) var. Petkus, which has rust genes such as Lr26, Sr31 andRr9. We designed primers based on the NBS region and performed PCR using Petkus genomic DNA a template. Next, we TA-cloned 532-bp fragment containing five homologous amino sequences in region. The SA-treated showed strong expression of transcript approximately 3.5 knt Northern blots probed with fragment; however, no transcripts were observed untreated rye. constructed cDNA library treated SA, then screened fragments probe. entire nucleotide sequence full length NBS-LRR-containing class 3,446 bp determined.
منابع مشابه
Identification and characterization of a NBS–LRR class resistance gene analog in Pistacia atlantica subsp. Kurdica
P. atlantica subsp. Kurdica, with the local name of Baneh, is a wild medicinal plant which grows in Kurdistan, Iran. The identification of resistance gene analogs holds great promise for the development of resistant cultivars. A PCR approach with degenerate primers designed according to conserved NBS-LRR (nucleotide binding site-leucine rich repeat) regions of known disease-resistance (R) gene...
متن کاملIdentification and characterization of a NBS–LRR class resistance gene analog in Pistacia atlantica subsp. Kurdica
P. atlantica subsp. Kurdica, with the local name of Baneh, is a wild medicinal plant which grows in Kurdistan, Iran. The identification of resistance gene analogs holds great promise for the development of resistant cultivars. A PCR approach with degenerate primers designed according to conserved NBS-LRR (nucleotide binding site-leucine rich repeat) regions of known disease-resistance (R) genes...
متن کاملidentification and characterization of a nbs–lrr class resistance gene analog in pistacia atlantica subsp. kurdica
p. atlantica subsp. kurdica, with the local name of baneh, is a wild medicinal plant which grows in kurdistan, iran. the identification of resistance gene analogs holds great promise for the development of resistant cultivars. a pcr approach with degenerate primers designed according to conserved nbs-lrr (nucleotide binding site-leucine rich repeat) regions of known disease-resistance (r) gene...
متن کاملcloning of three nucleotide-binding site leucine-rich repeat (nbs-lrr) class resistance gene analogs in pistacia khinjuk
pistacia khinjuk (stocks) is a native species that, along with p. atlantica, is widely distributed from eastern to western iran through the makran zone, zagros mountains and the sanandaj-sirjan zone, ranging from 50 to 3300 m above sea level. the identification of resistance gene analogs holds great promise for developing resistant plants. a pcr approach with degenerate primers designed from co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genetika
سال: 2021
ISSN: ['0016-6758']
DOI: https://doi.org/10.2298/gensr2101001t